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The images on this page are from posters we presented at FASEB (Fed Proc 38:1429,1979 & Fed Proc 39:698,1980). Jon Warren & I adapted an 'under agarose' neutrophil chemotaxis assay (Nelson et al J. Immunol. 115:1650, 1975) for use with lymphocytes isolated from rat thoracic duct lymph. Lymphocytes require 18 hours incubation to go the same distance that neutrophils go in 3 hours. In the absence of a "chemotactic gradient," TDL migrate about 125 micrometers compared to 750 micrometers for lymphocytes moving toward a gradient of 'acute phase' serum from rats inoculated with endotoxin. We now know that this serum contained mixtures of chemokines with very similar physical properties. We could not isolate "the" chemotactic factor which we now realize was a family that separated with molecule sizes less than 10KDa. It was especially exciting to be able to participate in the first study to implicate chemokines in leukocyte homing and emigration with Chris Larsen, Claus Zachariae and Kouji Matsushima of J. Oppenheim's laboratory (Science 243:1464,1989). You can access additional images and information by clicking on each picture and the "links" below.
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