Olivier BOUTAUD, Ph.D.

4505 Idaho avenue

Nashville, Tennessee 37209 U.S.A.

(615) 298-2756

PROFESSIONAL ADDRESS

Vanderbilt University

Department of Pharmacology

514 Robinson Research Building

Nashville, Tennessee 37232-6602 U.S.A.

Phone : (615) 343-7398 Fax : (615) 322-4707

EMAIL oliver.boutaud@mcmail.vanderbilt.edu

Training


1991 : D.E.A. in Pharmacology (equivalent to Advanced Graduate Degree in Pharmacology). (Louis Pasteur University, Strasbourg, France)

1994 : Ph.D. in Molecular and Cellular Pharmacology with a fellowship from the Ministry of Research and Technology. (Louis Pasteur University, Strasbourg, France)


1994-96: Postdoctoral fellowship in the laboratory of F. P. Guengerich, Biochemistry Department, Vanderbilt University, Nashville, TN

1996-97 : Postdoctoral fellowship in the laboratory of A. R. Brash, Pharmacology Department, Vanderbilt University, Nashville, TN

1997-98 : Research Fellow in the laboratory of J. A. Oates, Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN

1998-99 : Research Instructor in the laboratory of J. A. Oates, Division of Clinical Pharmacology, Vanderbilt University, Nashville, TN

Publications:

Boutaud, O. & Brash, A.R. (1999) Purification and Properties of the two Domains of the Allene Oxide Synthase-Lipoxygenase Fusion Protein of the Coral Plexaura homomalla. J. Biol. Chem., 274, 33764-33770.

Brame C. J., Boutaud, O., Oates J. A., Morrow, J.D., Salomon R. G., and Roberts, L. J. II, (1999) LC/MS/MS identification of the protein adducts formed by g-ketoaldehyde arachidonate oxidation products. Proc. 47th Ann. Conf. Mass. Spectrom. Allied Topics, (in Press).

Boutaud, O. , Brame C. J., Salomon R. G., Roberts, L. J. II, and Oates J. A. (1999) Characterization of the lysyl adducts formed from prostaglandin H2 via the levuglandin pathway. Biochemistry, 38, 9389 -9396.

Brash, A.R., Koljak, R., Boutaud, O. , Shieh, B-H. & Samel, N. (1999). Allene Oxide Synthesis by a Natural Fusion Protein with Lipoxygenase and Catalase-Related Domains. In Essential Fatty Acids and Eicosanoids, (Riemersma R.A., ed.) AOCS Press, Champaign, IL, pp 386-389.

Koljak, R., Boutaud, O. , Shieh, B-H., Samel, N. & Brash, A.R.(1997). Identification of a Naturally Occurring Peroxidase-Lipoxygenase Fusion Protein. Science, 277, 1994-1996.

Boutaud, O. , Ceruti, M., Cattel, L. & Schuber, F. (1995). Retention of the label during the conversion of [3-H-3]squalene into (3S)-2,3-oxidosqualene catalyzed by mammalian squalene oxidase. Biochem. Biophys. Res. Commun. 208(1), 42-47.

Viola, F., Brusa, P., Balliano, G., Ceruti, M., Boutaud, O. , Schuber, F., & Cattel, L. (1995). Inhibition of 2,3-oxidosqualene cyclase and sterol biosynthesis by 10- and 19-azasqualene derivatives. Biochem. Pharmacol. 50(6), 787-796.

Boutaud, O. , Dolis, D., & Schuber, F. (1992). Preferential Cyclization of 2,3(S)-22(S),23-Dioxidosqualene by Mammalian 2,3-Oxidosqualene-Lanosterol Cyclase. Biochem. Biophys. Res. Commun., 188(2), 898-904.

Communications at meeting:

"Effets d'un inhibiteur de l'époxyde de squalène-lanostérol cyclase sur la régulation de la biosynthèse du cholestérol dans les cellules HepG2"

Danièle Dolis, Olivier Boutaud et Francis Schuber

6ème symposium de Créteil : Nutrition, Lipides et Lipoprotéines (April 1992).

"Labeling of the active site of human recombinant cytochromes P450 1A2 and 3A4 using mechanism-based and photoactivatable inhibitors"

Hoffman-La Roche Switzerland (April, 15th 1996)

"Mechanism of inhibition of cytochrome P450 1A2 by 2-ethynylnaphthalene"

Olivier Boutaud and F. Peter Guengerich

Center in Molecular Toxicology Symposium : Enzymatic transformation of xenobiotic Chemical. (Nashville, April 1997)

“Protein-Adducts Formation by COX-I Catalyzed Arachidonic Acid Metabolites”.

Olivier Boutaud , Jemin Wang, Gwenn E. Sobo, Elizabeth A. Shipp, Cynthia J. Brame, John A. Oates.

19th annual meeting of the Southeastern Pharmacology Society (September, 9th-11th, 1998).

“LC/MS/MS Identification of Protein Adducts Formed by g-ketoaldehyde Arachidonate Oxidation Products”.

Cynthia J. Brame, Olivier Boutaud , John A. Oates, Jason D. Morrow, Robert G. Salomon, L. Jackson Roberts, II.

"The Antipyretic Action Of Acetaminophen: Inhibition Of The Cyclooxygenase Activity Of Endothelial Cells Treated With IL-1".

John A. Oates, Lawrence J. Marnett, and Olivier Boutaud .

11th International Conference on Advances in Prostaglandin and Leukotriene Research: Basic Science and New Clinical Applications (Florence, June 4th-8th, 2000).

Oxygenation Of Arachidonic Acid By Cyclooxygenases Generates Reactive Intermediates That Form Adducts With Proteins.

Olivier Boutaud , Junyu Li, Pierre Chaurand, Cynthia J. Brame, Lawrence J. Marnett, L. Jackson Roberts, II, and John A. Oates.

6th International Symposium on Biological Reactive Intermediates (Paris, July 16th-20th, 2000).

Nombre de visiteurs depuis le 22/12/99